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Imputation accuracy of bovine spongiform encephalopathy-associated PRNP indel polymorphisms from middle-density SNPs arrays
BSE cattle indel prion gene
2015/7/3
Statistical methods of imputation allow predicting genotypes of markers (which were not genotyped in the whole population) based on known linkage disequilibrium relationships between the flanking poly...
Relationship between the insertion/deletion polymorphism within the promoter and the intron 1 sequence of the PRNP gene and milk performance traits in cattle
Polish Holstein-Friesian cattle PRNP polymorphism milk performance traits QTL marker
2015/6/9
Current studies on the prion protein (PRNP) gene polymorphism focus primarily on the causative mutations that influence BSE susceptibility in cattle. The specific genetic structure determined by the i...
牛羊朊蛋白基因（PRNP）多态性与抗病性的研究进展
朊蛋白基因（PRNP） 插入/缺失多态 疯牛病抗性
2012/2/15
朊蛋白（PRNP）是近年来造成人和部分哺乳动物传染性海绵状脑病(TSE)的主要根源，该基因的多态性显著影响了人和动物对TSE的易感性或抗病性。本文分析了朊蛋白基因及其编码蛋白的结构与功能；简要介绍了绵羊基因编码区突变与致病性的关系；系统分析了牛科动物启动子区域内23bp的插入/缺失、第一内含子区域内12bp的插入/缺失及其与疯牛病（BSE）抗病性的作用机制；全面总结了全球已经报道的牛科动物12和2...
重组绵羊pEGFP-PRNP质粒构建及真核细胞转染
真核细胞转染 pEGFP-PRNP质粒构建 重组绵羊
2008/4/10
构建了携带绵羊朊蛋白基因（PRNP）的重组真核转染质粒，通过脂质体转染试剂将其转染至神经胶质瘤细胞（C6），以期为进一步研究绵羊朊蛋白的生理功能和从细胞水平研究朊蛋白疾病的发病机制奠定基础。采用PCR扩增目的基因序列，纯化后将其克隆到带有增强型绿色荧光蛋白（EGFP）报告基因的真核表达载体pEGFP-N1中，对重组质粒pEGFP-PRNP做酶切鉴定。而后采用阳离子脂质体转染法将重组质粒转染到C6细...
PRNP基因八肽重复突变研究进展
遗传性朊病毒病 八肽重复突变
2007/11/5
摘要 摘要 遗传性朊病毒病由编码PrP蛋白的PRNP基因突变引起，其中八肽重复区的插入或缺失突变与家族性克雅氏病（fCJD）、GSS综合症相关。PRNP基因八肽重复突变的细胞生物学、转基因动物研究对揭示遗传性朊病毒病发病机制，进而揭示朊病毒病的致病机理具有重要意义。
Genomic Sequence Variability of the Prion Gene (PRNP) in Korean Cattle
PRNP SNP BSE Indel Korean Cattle
2016/4/25
In this study, we have investigated sequence variants in the PRNP gene of 20 individuals belonging to the Korean cattle, and have analyzed and compared genetic features between varieties of other catt...